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| | FabD/FabH Coupled Assay |
| Description: | The potency of FabH inhibition (IC50) was determined using [3H]- or [14C]-radiolabeled substrates. This was accomplished at fixed concentrations of acetyl-CoA in a coupled FabD/FabH assay system that generates the substrate malonyl-ACP in situ. Subsequently, the FabH acetylation reaction was performed to exclude the possible contribution of undesired FabD inhibitory activity. The incorporation of the 3H (or [14C]) signal in the final product was read by liquid scintillation. |
| Affinity data for this assay | |
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