new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  FabD/FabH Coupled Assay
Description:  The potency of FabH inhibition (IC50) was determined using [3H]- or [14C]-radiolabeled substrates. This was accomplished at fixed concentrations of acetyl-CoA in a coupled FabD/FabH assay system that generates the substrate malonyl-ACP in situ. Subsequently, the FabH acetylation reaction was performed to exclude the possible contribution of undesired FabD inhibitory activity. The incorporation of the 3H (or [14C]) signal in the final product was read by liquid scintillation.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.