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Assay Method Information

Assay Name:  Kinesin ATPase In Vitro Assay
Description:  The kinesin motor domain is incubated with microtubules, 1 mM ATP (1: 1 MgCl2 : Na-ATP), and compound at 23°C in buffer. After reaction was terminated, free ph osphate from the ATP hydrolysis reaction is measured via a quinaldine red/ammoni um molybdate assay. The absorbance of the phospho-molybdate complex is measured Human poly-histidine tagged KSP motor domain was cloned, and protein was expressed in E. coli.
Affinity data for this assay
 

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Last update November 1, 2007
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