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Assay Method Information

Assay Name:  HDAC Inhibition Assay
Description:  The inhibitory effects of compounds on histone deacetylase (HDAC) activity were determined using a fluorescence-based assay with electrophoretic separation of substrate and product carried out using a microfluidic system followed by quantitation of fluorescence intensity in the substrate and product peaks. IC50 values were calculated using the IDBS XLFit version 4.2.1 plug-in for Microsoft Excel and the XLFit 4 Parameter Logistic Model (Sigmoidal Dose-Response Model).
Affinity data for this assay
 

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Last update November 1, 2007
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