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Assay Method Information |
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| | DNA Gyrase ATPase Assay |
| Description: | Enzymatic hydrolysis of ATP to ADP was coupled to the conversion of NADH to NAD+. The decrease in NADH absorbance was monitored at 340 nm for 20 min with a Molecular Devices plate reader. The rate of enzymatic hydrolysis was plotted against a serial dilution of inhibitor to determine potency, and the data were fitted to the Morrison equation for tight-binding inhibition. |
| Affinity data for this assay | |
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