BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  HDAC Inhibition Assay
Description:  HDAC activity was measured using a continuous trypsin-coupled assay. For inhibitor characterization, measurements were done using 96-well assay plates. Enzyme was mixed with inhibitor at various concentrations and allowed to incubate for 15 minutes. Trypsin was added to initiate the reaction. Reactions were monitored in a fluorescence plate reader. After a 30-minute lag time, the fluorescence was measured over a 30-minute time frame using an excitation wavelength of 355 nm and a detection wavelength of 460 nm. The increase in fluorescence with time was used as the measure of the reaction rate. Inhibition constants Ki(app) were obtained using the program BatchKi.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.