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Assay Method Information |
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| | CDK2/CyclinA Kinase Assay |
| Description: | CDK2/cyclin A activity was determined using a radiometric assay to measure the incorporation of gamma-phosphate from [gamma-33P]-ATP into histone H1. After the reaction, phosphorylated histone H1 was separated from excess ATP. Incorporated radioactivity was counted using Packard TopCount. IC50 values were determined using a sigmoidal dose response equation from Prism GraphPad Software. |
| Affinity data for this assay | |
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