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Assay Method Information |
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| | TrkA Enzyme Assay |
| Description: | The kinase domain of the human TrkA receptor in phosphorylation buffer with 0.5 uM ATP is incubated in plates coated with PGT substrate. Compounds are incubated for 30 min and the plates are washed. The amount of phosphorylated PGT is quantitated by incubation with HRP-conjugated PY-54 antibody, and developed with ABTS substrate. |
| Affinity data for this assay | |
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