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| | Mixed-Lineage Kinase Assay |
| Description: | The MLK kinase assays were performed by using the Millipore multiscreen trichloroacetic acid (TCA) in-plate format. The inhibition curves were generated for the compounds by plotting percent control activity versus log10 of the concentration of compound. The IC50 values were calculated by nonlinear regression using the sigmoidal dose-response (variable slope) equation in GraphPad Prism. IC50 values were reported as the average of duplicates that agree within 20%. |
| Affinity data for this assay | |
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