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Assay Method Information

Assay Name:  Kinase Inhibition Assay
Description:  IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-33P] labeled ATP to a protein or peptide substrate. Use of 33P-labeled ATP allows this transfer to be monitored by scintillation counting or by autoradiography of the substrate.
Affinity data for this assay
 

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Last update November 1, 2007
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