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Assay Method Information

Assay Name:  Scintillation Proximity Assay
Description:  Test compounds were added to reaction buffer containing yttrium silicate (Ysi) polylysine SPA beads (Amersham), and the enzyme was added to the assay mixture to initiate the kinase reaction. After incubation, the mixture was centrifuged and read using a Wallac Microbeta counter (Perkin-Elmer). The reported IC50 values are means of at least 2 independent experiments and are calculated using a sigmoidal, dose-response curve fit of 4 parameter logistic model using MDL Assay Explorer.
Affinity data for this assay
 

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Last update November 1, 2007
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