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Assay Method Information

Assay Name:  PI3Kgamma Inhibition Assay
Description:  PI3Kgamma activity was assessed by incubation of baculoviral co-expressed regulatory and catalytic subunits (p101 and p110) with lipid micelles prepared from phosphatidylethanolamine containing phosphatidylinositol 4,5 bisphosphate (PIP2) at a molar ratio of 8:1. Recombinant G-protein beta gamma-subunits were used to activate the enzyme complex. The formation of 32P-PIP3 on the filters was then monitored by scintillation counting. IC50 values were determined by incubation of the compound at various concentrations (typically a 10 point half-log dose distribution) and curve fitting by using GraphPad Prism.
Affinity data for this assay
 

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Last update November 1, 2007
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