Assay Method Information |
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| In Vitro BRAF Kinase Assay |
Description: | BRAF kinase activity was quantified using an ELISA-based MEK phosphorylation assay. IC50 values were derived from the sigmoidal dose-response curve fitting by GraphPad Prism 4.0. Ki was calculated from the Cheng-Prusoff equation. |
Affinity data for this assay | |
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