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| | beta2 Adrenergic Receptor Binding Assay |
| Description: | The displacement of [125I]-(-)iodopindolol binding from the beta2AR was determined using DDT1 MF-2 cell membranes. Nonspecific binding was determined in parallel assays that contained 1 uM alprenolol. The radioactivity was determined in a gamma counter. All assays were performed in triplicate. The concentration of compounds that inhibited radioligand binding by 50% (IC50) was obtained by nonlinear regression analysis using GraphPad Prism. The dissociation constant (Ki) was obtained from IC50 value by using the conversion described by Cheng and Prusoff. |
| Affinity data for this assay | |
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