new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  B-Raf Activity Assay
Description:  Activity of human recombinant B-Raf protein was assessed in vitro by assay of the incorporation of radiolabeled phosphate to recombinant MAP kinase (MEK). To ensure that all substrate phosphorylation resulted from B-Raf activity, a catalytically inactive form of MEK was utilized. The assay was initiated by the addition of ATP. Following the incubation, the assay mixtures were quenched, and the product was captured on a Perkin-Elmer GF/B filter plate using a Tomtec Mach III Harvester. The incorporated radioactivity was counted in a Topcount NXT (Packard)
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.