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| | Ligand Binding Assay |
| Description: | The assay was developed based on the optical spectral property of P450 enzyme to elicit both type I and type II spectral changes (350 nm through 450 nm). The concentration dependence of the changes allows the binding affinities of the ligands to be estimated. Titration data were linearized by plotting S0/deltaA against S0, where S0 is the total concentration of a ligand and deltaA is the change in absorption. The equilibrium dissociation constant (Kd) was extrapolated from the intercept of the linear plot with the S0 axis. |
| Affinity data for this assay | |
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