new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Ligand Binding Assay
Description:  The assay was developed based on the optical spectral property of P450 enzyme to elicit both type I and type II spectral changes (350 nm through 450 nm). The concentration dependence of the changes allows the binding affinities of the ligands to be estimated. Titration data were linearized by plotting S0/deltaA against S0, where S0 is the total concentration of a ligand and deltaA is the change in absorption. The equilibrium dissociation constant (Kd) was extrapolated from the intercept of the linear plot with the S0 axis.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.