Assay Method Information |
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| LXR Binding Assay and hLXR Reporter Assay |
Description: | Binding reaction was initiated by adding a dilution series of test compound in tracer solution to LXR-coated flash plates. Each concentration of test compound was analyzed in duplicate. Bound radioactivity was measured in a Wallac Microbeta normalized for flashplates. Huh-7 human hepatoma cell based Gal4 transactivation assays were used to assess the agonist potency and efficacy. EC50 values were determined from dose response curves represented measurement at 12 concentrations, each concentration was measured in quadruplicates. Efficacy was measured relative to T0901317. |
Affinity data for this assay | |
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