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Assay Method Information

Assay Name:  FDH Coupled Inhibition Assay
Description:  A coupled-assay for JMJD2E activity employing formaldehyde dehydrogenase (FDH) from Pseudomonas putida was developed. Formaldehyde release by demethylation of the histone peptide substrate was monitored by its oxidation to give formate as catalyzed by FDH, which is carried out concomitantly with the reduction of NAD+. The production of NADH was monitored by fluorescence spectroscopy.
Affinity data for this assay
 

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Last update November 1, 2007
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