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| | Radioligand Binding Assay |
| Description: | Membrane homogenates were incubated at room temperature with a sub-Kd concentration of radioligand in the absence or presence of seven different concentrations of unlabeled ligand. Data were fit by nonlinear regression to a one-site competition curve. IC50 is the concentration of unlabeled ligand that inhibits 50% of [3H]ligand binding (Prism, GraphPad Software). Equilibrium dissociation constant values for the unlabeled ligand (Ki) were calculated using the Cheng-Prusoff-Chou equation. |
| Affinity data for this assay | |
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