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Assay Method Information |
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| | MurD Inhibition Assay |
| Description: | The compounds were tested for their ability to inhibit the addition of D-[14C]Glu to UMA. The radioactive substrate and product were separated by reverse-phase HPLC. The compounds were detected and quantified with an LB 506 C-1 HPLC radioactivity monitor using a scintillator. The residual activities for each of the inhibitor concentrations were calculated with respect to a similar assay without inhibitor. The values are expressed as the mean of two independent experiments, and standard deviations were within +/-10% of the mean values. The IC50 values were calculated from the fitted regression equation using the logit-log plot. |
| Affinity data for this assay | |
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