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Assay Method Information

Assay Name:  FAAH Competitive ABPP Using FP-Rhodamine
Description:  Mouse brain membrane proteomes were preincubated with varying concentrations of inhibitors for 10 min prior to the addition of a rhodamine-tagged fluorophosphonate (FP-rhodamine). Reactions were quenched after 10 min, subjected to SDS-PAGE, and visualized in-gel using a flatbed fluorescence scanner. Labeled proteins were quantified by measuring integrated band intensities. Dose response curves obtained from three trials at each inhibitor concentration were fit using Prism software (GraphPad) to
Affinity data for this assay
 

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Last update November 1, 2007
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