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Assay Method Information

Assay Name:  FAAH Inhibition Assay
Description:  FAAH Inhibition Assay [3H]Ethanolamine produced from [3H]AEA hydrolysis was used to calculate FAAH activity and was measured by scintillation counting of the aqueous phase after extraction of the incubation mixture. Data were expressed as the percentage of [3H]-ethanolamine formed versus vehicle, after subtraction of the background radioactivity determined in the presence of arachidonyl trifluoromethyl ketone (ATFMK), an inhibitor of FAAH. IC50 values were determined using a four-parameter logistic equation for dose-response curves.
Affinity data for this assay
 

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Last update November 1, 2007
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