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Assay Method Information |
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| | CDK Inhibition Assay |
| Description: | In vitro kinase assay using purified enzyme, was incubated at 30 °C with substrate, and test compounds in the presence of 15 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a scintillation counter. IC50 values were calculated from dose-response curves. |
| Affinity data for this assay | |
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