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Assay Method Information

Assay Name:  PARP-1 Enzyme Assay
Description:  A FlashPlate scintillation proximity assay has been developed to identify inhibitors of PARP-1. The mechanism of action of the assay requires the binding of PARP-1 to a double-stranded DNA oligonucleotide leading to the active enzyme. Using NAD+/[3H]NAD+ as substrate, activated PARP-1 synthesizes labeled poly(ADP-ribose) chains. Once the reaction is stopped, ADP-ribose polymers are brought into proximity with the pretreated FlashPlate walls, resulting in signal amplification. This signal is then detected by a TopCount scintillation plate reader. All experiments were repeated at least three times.
Affinity data for this assay
 

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Last update November 1, 2007
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