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| | PARP Enzyme Inhibition Assay |
| Description: | To assess the inhibitory activity of novel inhibitors, the PARP enzyme assay was carried out in reaction mixture consisting of activated salmon testes DNA, [adenylate-32P]-NAD, recombinant PARP enzyme, and various concentrations of PARP inhibitors. The reaction mixture was incubated at room temperature for 15 min, and the reaction was terminated by adding trichloroacetic acid. The precipitate was transferred onto GF/B filter. After the filter was washed and dried, the radioactivity was determined by liquid scintillation counting. IC50 values were calculated from the concentration dependence of the inhibition curves by using computer-assisted non-linear regression analyses. |
| Affinity data for this assay | |
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