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Assay Method Information

Assay Name:  IGF-1R Inhibition Assay
Description:  Assays are performed in 384-well microtiter plates in reaction buffer containing biotinylated substrate, ATP, and purified activated GST-IGF-1R in the presence of test compounds. After incubation, Streptavidin-APC and Europium conjugated phospho-tyrosine antibodies (Perkin-Elmer) are added for signal detection. The signal is read on PerkinElmer Viewlux microplate imager or Wallac Victor fluorometer.
Affinity data for this assay
 

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Last update November 1, 2007
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