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Assay Method Information

Assay Name:  Fluorescence Polarization Affinity Measurements
Description:  Samples for fluorescence polarization affinity measurements were prepared by addition of serial dilutions of target protein to each well containing antagonist and polarization probe in the buffer. Samples were read after 30-min incubation. Fluorescence polarization values were plotted as a function of the antagonist concentration, and the IC50 values were obtained by fitting the data to a four-parameter equation using KaleidaGraph software. Ki values for the antagonists were determined from the IC50 values.
Affinity data for this assay
 

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Last update November 1, 2007
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