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Assay Method Information |
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| | Protease Inhibition Assay |
| Description: | A fluorimetric assay was used to determine the effects of the inhibitors on HIV-1 protease. This assay used an internally quenched fluorescent peptide substrate. The measurements were performed in 96-well plates with a Fluoroscan plate reader. Excitation and emission wavelengths were 355 nm and 500 nm, respectively. |
| Affinity data for this assay | |
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