Assay Method Information |
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| FXR Co-transfection Assay |
Description: | The assays were performed using CV-1 African Green Monkey Kidney cells transfected with the vectors harboring human FXR, RXR, and luciferase reporter genes. The co-transfection assays were used to establish the EC50 values for potency and percent activity for efficacy. Efficacy defines the activity of a compound relative to a high control (100 uM of chenodeoxycholic acid) or a low control (DMSO/vehicle). The dose response curves are generated from an 8 point curve with concentrations differing by 1/2LOG units. Each point represents the average of 4 wells of data from a 384 well plate. The EC50 values represented are the averages of at least 3 independent experiments. |
Affinity data for this assay | |
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