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Assay Method Information

Assay Name:  Radioligand Binding Assay (Ki)
Description:  Competition experiments were performed in the presence radioligand with membrane protein (obtained from cells expressing the receptor) and test compounds. The mixture was incubated at room temperature for 60 min and the reaction stopped by rapid filtration onto a 96-well GF/B filter using a Packard Filtermate harvester. Radioactivity retained on the filters was measured by liquid scintillation in a Packard TopCount. IC50 values for test compounds were determined from nonlinear regression analysis of data collected from ligand binding experiments. The inhibition constant (Ki) was calculated from IC50 value by the Cheng and Prusoff equation.
Affinity data for this assay
 

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Last update November 1, 2007
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