Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Transactivation Cell-Based Assay |
| Description: | The assay was to monitor the SF-1 activation state via co-transfection of CHO-K1 cells with a plasmid encoding a Gal4-SF-1 chimerical transcription factor, pGal4DBD-SF-1-LBD, and a second plasmid driving luciferase expression under control of 5 multimerized Gal4 binding sites. The assay began by dispensing transfected cell suspension to each well (4,000 cells / well) of a white solid-bottom 1536-well plate. One hour after seeding, cells were treated with test compounds or DMSO alone (high control). The -NR cells (not co-transfected with nuclear receptor) were treated with DMSO (low control). After incubation, the luciferase assay was performed, and light emission was measured for 30 seconds with the ViewLux reader (PerkinElmer, Turku, Finland). Activity of each compound was calculated using the equation: Percent inhibition of compound=100 x (1-(test well-low control)/ (high control-low control)) EC50/IC50 is the concentration of test compound to exert 50% inhibition. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||