new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Cell-Based Assay for Amyloid-beta Accumulation
Description:  H4 human neuroglioma cells expressing beta-APP were aliquoted into a 96-well plate, and after attachment the medium was replaced with the conditioned medium containing test compounds. After an overnight incubation, the plates were evaluated for the presence of amyloid-beta (A-beta) in a sandwich ELISA using a monoclonal C-terminal A-beta 40 specific capture antibody and an HRP labeled monoclonal antibody to the N-terminus of A-beta for detection. The plates were read at 450 nm. IC50s are means of two experiments, with 12 drug concentrations in each experiment; intra-assay variance was less than 10%
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.