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| | Cell-Based NOS Transient Transfection Assay |
| Description: | HEK293 cells (ATCC) were transiently transfected with recombinant NOS and grown. Cells were trypsinized, resuspended to 5 00,000 cells/mL, and plated in 1536-well plates, followed by immediate treatment with compound. After 18 h of incubation, NO production was measured by adding 2,3-diaminonaphthalene (DAN) and incubated for 20 min at room temperature. Fluorescence was measured after increasing pH with sodium hydroxide (0.12 N). |
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