new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Receptor Binding Assay (Ki) and Transactivation Assay (EC50)
Description:  Binding assays were conducted by incubating test compound at various concentrations with [3H]DHT with human cancer epithelial breast cell lines MDAMB-45. IC50 value was obtained from dose-response displacement curves. The inhibition constant (Ki) was calculated from IC50 value by the Cheng and Prusoff equation. EC50 value was obtained from functional transactivation assay, which was conducted to assess the potency and efficacy of agonist/antagonist in stably transfected mouse myoblast C2C12 cell line utilizing a luciferase reporter.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.