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| | Radioligand Binding Assay (Ki) |
| Description: | Competition experiments were performed in the presence radioligand with membrane protein and test compounds. After incubation, the reaction stopped by rapid filtration onto a 96-well GF/B filter. Radioactivity retained on the filters was measured by liquid scintillation counting (Beckman LS 6500 apparatus). IC50 values for test compounds were determined from nonlinear regression analysis of data collected from ligand binding experiments. The inhibition constant (Ki) was calculated from IC50 value by the Cheng and Prusoff equation. |
| Affinity data for this assay | |
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