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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The caspase enzymatic activity was determined by measuring the accumulation of a fluorogenic product. All assays were prepared in 96-well format. Recombinant caspase enzyme was first assayed to determine the optimal concentration for each experiment. Peptide inhibitors with known IC50 values were tested together with the compounds as a control for each caspase assay. After incubation, Amount of AMC released was determined by using a Victor microplate fluorometer (Perkin Elmer Life Sciences, Boston, MA) at excitation and emission wavelengths 355 nm and 460 nm, respectively. Compounds were tested in duplicate and IC50 curves were calculated for all inhibitors assayed. Final IC50 values were the average of 3 independent experiments.
Affinity data for this assay
 

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Last update November 1, 2007
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