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| | pfHDAC-1 Enzyme Assay |
| Description: | Recombinant pfHDAC-1 was assayed with substrate in the presence of test compound. The substrate concentration was kept constant at 125 uM while the candidate inhibitor concentration was varied from 0.01 nM to 10 uM. The reaction was allowed to proceed at room temperature for 15 hrs in sealed plates to minimize evaporation. The rate of product formation was determined to be linear over this time period. Fluorescence was monitored after 1 hr at excitation and emission wavelengths of 360 and 460 nm, respectively. Kinetic constants were calculated by non-linear regression fitting of the data to the Michaelis-Menten equation. Kinetic and inhibition values reported are the mean of three determinations +/- standard deviation. |
| Affinity data for this assay | |
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