Assay Method Information |
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| Enzyme Inhibition Assay |
Description: | Enzyme activity and inhibition were assayed using a fluorescent FAM-[SEVNLDAEFK]-TAMRA substrate. Control reactions with no enzyme were included in each assay. Quantification of substrate cleavage was assessed using an LJL analyst spectrophotometer (485nm excitation, 535nm emission). |
Affinity data for this assay | |
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