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| | 11beta-HSD1 Homogeneous Time-Resolved Fluorescence (HTRF) Assay |
| Description: | 11beta-HSD1 enzyme activity was assessed in buffer containing a substrate mixture cortisone/NADPH in the presence of test compound. Reaction was initiated by addition of 11beta-HSD1 and terminated with addition of 18beta-glycyrrhetinic acid stop solution. Determinations of cortisol levels were monitored by HTRF assay (Cisbio International). |
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