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| | MK-2 IC50 Value Determination |
| Description: | The phosphorylation of HSP27 peptide by MAPKAPK2 was measured using an anion exchange resin capture assay method. The reaction was carried out in reaction buffer containing enzyme and test compound in the presence of the HSP-peptide with [gamma-33P]ATP /ATP. After the reaction was terminated and [gamma-33P]ATP was removed from solution by the addition of AG 1X8 ion exchange resin. An aliquot was removed from the quenched reaction mixture and added to a 96-well plate. Microscint-40 (Packard) was added and the amount of phosphorylated-peptide was determined. |
| Affinity data for this assay | |
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