BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  hKAT-I Activity Assay
Description:  A typical reaction mixture contains kynurenine, alpha-ketobutyrate, PLP and hKAT-I in the presence of test compound. The reaction mixture was incubated for 20 min at 38 deg C, and the reaction was stopped by adding an equal volume of 1 M formic acid. The product KYNA was detected by high-performance liquid chromatography and quantified on the basis of the absorbance at 330 nm as measured by a Hitachi L-7400 UV detector. The assays for each inhibitor were performed four times, and the data were analyzed using the SigmaPlot enzyme kinetics module (SPSS Inc.).
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.