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Assay Method Information

Assay Name:  HSP70 Fluorescence Polarization Assay
Description:  A fluorescein-labeled ATP-based probe was utilized, which binds to the GST fused ATPase domain (amino acids 3-383) of HSP70, producing an increase in mP. The presence of a compound which competitively binds to the same site as the probe would lead to decreased mP values, by increasing the amount of free probe. Compounds were tested as 10-point IC50s. Assay mixtures were incubated for three hours prior to reading on the Fusion (ex 485 nm; em 535 nm). The data was fitted using a 4 parameter logistical data model by XLFit 4 (IDBS).
Affinity data for this assay
 

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Last update November 1, 2007
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