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| | Enzyme Inhibition Assay |
| Description: | Inhibitors were assayed in a 96-well plate format. Typically, protease was preincubated with concentrations of test compounds at 37 deg C for 30 min. The reaction was initiated by the addition of substrate Bz-nKRR-AMC. Reaction progress was monitored continuously by following the increase in fluorescence on a Tecan Safire2 plate reader. IC50 values of inhibitors were derived by fitting the calculated initial velocities to a nonlinear regression curve using GraphPad Prism software. Each point of the IC50 curve was measured in duplicate during a single experiment. |
| Affinity data for this assay | |
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