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| | Protease Inhibition Assay |
| Description: | All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spectrofluorometer. Three different concentrations of inhibitors were used for calculating Ki values. Kinetic data were measured with an excitation wavelength of 390nm and emission wavelength of 460nm. From the initial velocities of the reactions Ki values were calculated by nonlinear regression method using the program EZ-fit (Perella Scientific). |
| Affinity data for this assay | |
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