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Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  Ki values were determined by using a fluorescent substrate (DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS). All incubations were performed at 30°C in 0.1 M sodium acetate 1 M NaCl 1 mM DTT 1 mM EDTA 3% DMSO (pH 5.0) by using 5 uM substrate and the indicated enzyme concentrations ([E]). The enzyme concentration (A280) was determined by absorption measurements. Km values, including standard deviations, and calculated values of the active enzyme concentrations were obtained by nonlinear regression analysis.
Affinity data for this assay
 

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Last update November 1, 2007
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