Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | GTP-gamma-S Binding Assay |
| Description: | A GTP-gamma-S CB1 functional assay was used to assess the potency of the pyrazine derivatives. [35S]GTP-gamma-S binding assays were performed at 30 deg C fro 45 min in membrane buffer containing membrane protein, GDP and [35S]GTP-gamma-S in the presence of test compounds. Non specific binding was determined in the presence of 20 uM GTP-gamma-S. The reaction was terminated by addition of ice cold wash buffer followed by rapid filtration under vacuum through Printed Filtermat A glass fibre filters (Wallac) using a Micro 96 Harvester. The bound radioactivity was determined using a 1450 Microbeta Trilux (Wallac) scintillation counter. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||