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Assay Method Information

Assay Name:  QFRET-based counterscreen for inhibitors of PFM18AAP: biochemical high throughput dose response assay for inhibitors of the Cathepsin L proteinase (CTSL1).
Description:  Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: John Dalton and Donald Gardiner, Queensland Institute of Medical Research, Australia Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH084103-01 Grant Proposal PI: John Dalton and Donald Gardiner, Queensland Institute of Medical Research, Australia External Assay ID: CTSL1_INH_QFRET_1536_3XIC50 Name: QFRET-based counterscreen for inhibitors of PFM18AAP: biochemical high throughput dose response assay for inhibitors of the Cathepsin L proteinase (CTSL1). Description: Aminopeptidases (APs) are metalloproteases that cleave amino-terminal (N-terminal) amino acids during protein synthesis (1, 2) . These enzymes are characterized in part by their post-translational removal of leucine, aspartate, proline, methionine, etc from proteins and peptides, in order that proteins are properly regulat
Affinity data for this assay
 

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Last update November 1, 2007
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