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Assay Method Information

Assay Name:  Absorbance Microorganism-Based Dose Retest to Identify Inhibitors of Vibrio harveyi
Description:  Keywords: Quorum Sensing, Auto-inducer 2 (AI-2), Vibrio harveyi, LuxS, LuxPQ, Luminescence Assay Overview: A modified strain of Vibrio harveyi with only the AI-2 quorum sensing system intact will be exposed to small molecules. Growth of the organism post exposure will be followed using optical density and disruption of quorum sensing will be observed based on decreased luminescent signal. Expected Outcome: Identification of AI-2 quorum sensing system inhibitors with modes of action which either antagonize the LuxPQ receptor or inhibit LuxS synthase. Such inhibitors should not perturb growth (observed by optical density) but should perturb quorum sensing (observed by luminescence)
Affinity data for this assay
 

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Last update November 1, 2007
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