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Assay Method Information |
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| | FabH Enzyme Assay |
| Description: | Escherichia coli FabH assays were carried out using standard coupled trichloroacetic acid precipitation assay to determine the rate formation of radiolabeled 3-ketoacyl-ACP from malonyl-ACP. Mycobacterium tuberculosis FabH assay was done by sustituting malonyl-CoA for MACP. |
| Affinity data for this assay | |
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