new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  SAR Selectivity Analysis of small molecule inhibitors of PEST using pCAP in a fluorescence assay
Description:  Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network(MLPCN) Grant Number: 1R21NS056945-01 Assay Provider: Dr. Nunzio Bottini, La Jolla Institute for Allergy and Immunology, La Jolla, CA PTP-PEST, encoded by PTPN12 gene, is a protein tyrosine phosphatase that contains a C-terminal PEST motif, which serves as a protein-protein interaction domain. This PTP dephosphorylates c-ABL, and may play a role in oncogenesis. This is a selectivity counterscreen to aid with identification of specific inhibitors of LYP phosphatase (AID 2135). This assay uses a phosphorylated coumarin amino acid (pCAP) moiety attached to a 14-mer peptide, which is a substrate for LYP. This dose response assay is developed and performed to assess selectivity of hits originally found in "uHTS identification of small molecule inhibitors of LYP via a fluo
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.