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Assay Method Information

Assay Name:  Dose Response confirmation of small molecule APOBEC3G DNA Deaminase Inhibitors via a fluorescence-based single-stranded DNA deaminase assay
Description:  Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH089432-01 Assay Provider: Dr. Reuben S Harris, Regents of the University of Minnesota, Minneapolis, MN The human APOBEC3G (A3G) protein is a DNA deaminase that has potent activity against HIV-1 and a variety of other retroelements. Although HIV encodes a natural inhibitor of A3G called Vif, it was hypothesized that the virus still benefits from the mutagenic activity of A3G (immune escape and drug resistance). The purpose of the screening is to identify A3G inhibitor by using a fluorescence-based single-strand DNA deaminase assay. In this assay, an A3G protein, a single-strand DNA oligonucleotide with a 5'-CCC target site, and Uracil DNA Glycosylase (UDG) are all mixed. After incubation at room temperature, A3G deaminates C-to-U
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